Ligand-based virtual screening (LBVS) & ligand-based drug discovery (LBDD).

Target-based virtual screening (TBVS) & target-based drug discovery (TBDD).

Biochemical assays for enzymatic inhibitors based on spectrophotometry and biocalorimetry.

Enzyme kinetics and biothermodynamics of drugs to determine the mode of action and the ligand-macromolecule interaction.

Molecular biology: cloning, expression, isolation and purification of T. cruzi enzymes.

Cell-based assays: in vitro models to determine the pharmacological activity and toxicity of antiparasite and anti-cancer compounds.

Systems chemical biology as a new concept to develop the integration of biological and chemical knowledge on a medicinal chemistry & drug discovery endeavor.